مشخصات پژوهش

A new and sensitive CL method was proposed for determination of paracetamol (PCM) and isoniazid (IND) in pharmaceutical preparations. The CL method is based on enhancing e ect of IND and also decreasing e ect of PCM on the graphene oxide (GO)-luminol-dissolved oxygen (DO) chemiluminescence (CL) reaction in a strongly alkaline medium. GO was synthesized by using improved hummer method and characterized by X-ray di raction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and Fourier-transform infrared spectroscopy (FTIR) methods. The e ects of various chemical reagents, involved in the CL reaction, on the CL emission intensity were also investigated and optimized. Under the optimum conditions, a linear re- lationship was obtained between the CL intensity and the concentration of PCM and IND in the range of 7.0 × 10−6–5.0 × 10−5 mol L−1 and 1.0 × 10−7–1.0 × 10−4 mol L−1 with limit of detections of 8.7 × 10−7 and 7.9 × 10−8 mol L−1, respectively. The mechanism of CL system was also discussed, brie y.