کلیدواژهها
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D2-D3, Description, Molecular analysis, Morphology, Morphometrics,
Taxonomy.
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چکیده
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Two species of the genus Pungentus, one new and one known,
collected in natural vegetation and cultivated soils in northwest of
Iran, are studied. Pungentus sufiyanensis n. sp. is characterized by
its 1.22 to 1.57 mm long body, offset lip region by a constriction
and 7 to 9 μ m broad, 18 to 21 μ m long odontostyle, 304 to
348 μ m long neck, 133 to 161 μ m long esophageal expansion,
mono-opisthodelphic female genital system without anterior uterine
sac, slightly backward directed vagina, absence of pars refringens
vaginae, V = 47−54, rounded-conoid caudal region (17.5–23 μ m,
c = 65–84, c´ = 0.7–1) with saccate bodies, and the absence of male.
Molecular analysis, based on D2-D3 expansion segments of the
28S rDNA (LSU), confirms the monophyly of the family Nordiidae
and suggests the monophyly of the genus Pungentus, with the
new species forming a clade with other Iranian species. New data
are presented for six Iranian populations of P. engadinensis, and
an updated key for the identification of Pungentus species is also
provided.
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