عنوان مجله
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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چکیده
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L-asparaginase is an enzyme capable of hydrolyzing the asparagine to aspartic acid and ammonia. L-asparaginase
is widely used in the treatment of acute lymphoblastic leukemia (ALL) and other cancers. Here, for the first time,
the effects of a novel yeast L-asparaginase from Yarrowia lipolytica were studied on human lung (A549) and
breast cancer (MCF7) cell lines as the solid cancer cell lines in terms of cell growth and metastasis inhibition.
Functional analysis showed the L-asparagine deprivation mediated anti-proliferation effects by apoptosis induction and changes in the expression of target genes involved in apoptosis and migration pathways. The qRT-PCR
analysis showed the higher expression levels of pro-apoptosis genes, including Bax, P53, caspase 3, caspase 8, and
down-regulation of Bcl-2 anti-apoptotic gene in treated cells. On the other hand, there was no increase in ROS
production in the treated cells. However, L-asparaginase treatment was able to significantly induce autophagy activation in A549 cells. Besides, wound healing assay showed that L-asparaginase could considerably inhibit the
migration of A549 and MCF7 cells. Taken together, our results suggested that Yarrowia lipolytica L-asparaginase
might be considered for enzyme therapy against breast and lung cancers
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