عنوان مجله
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Computational and Structural Biotechnology Journal
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چکیده
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Salt stress is an important factor limiting plant productivity by affecting plant physiology and metabolism.
To explore salt tolerance adaptive mechanisms in the model legume Medicago truncatula, we used
three genotypes with differential salt-sensitivity: TN6.18 (highly sensitive), Jemalong A17 (moderately
sensitive), and TN1.11 (tolerant). Cellular damage was monitored in roots and leaves 48 h after
200 mM NaCl treatment by measuring lipid peroxidation, nitric oxide, and hydrogen peroxide contents,
further supported by leaf stomatal conductance and chlorophyll readings. The salt-tolerant genotype
TN1.11 displayed the lowest level of oxidative damage, in contrast to the salt sensitive TN6.18, which
showed the highest responses. Metabolite profiling was employed to explore the differential genotyperelated
responses to stress at the molecular level. The metabolic data in the salt tolerant TN1.11 roots
revealed an accumulation of metabolites related to the raffinose pathway. To further investigate the sensitivity
to salinity, global transcriptomic profiling using microarray analysis was carried out on the saltstressed
sensitive genotypes. In TN6.18, the transcriptomic analysis identified a lower expression of many
genes related to stress signalling, not previously linked to salinity, and corresponding to the TIR-NBS-LRR
gene class. Overall, this global approach contributes to gaining significant new insights into the complexity
of stress adaptive mechanisms and to the identification of potential targets for crop improvement.
2021 The Author(s). Published by Elsevier B.V. on behalf of Research Network of Computational and
Structural Biotechnology. This is an open access article under the CC BY-NC-ND license
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