چکیده
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This study represents synthesis, characterization,
screening of antibiofilm efficacy, and cytotoxicity
of iridium bis-terpyridine complexes. The
complexes were characterized by NMR, MS, FTIR,
UV/Visible, and fluorescence spectroscopies. The
efficacy of biofilm inhibition and eradication of
iridium complexes was evaluated using a crystal
violet assay test and verified by fluorescence microscopy.
Cytotoxicity and apoptosis analysis of iridium
complexes were determined in this study. The results
of our study revealed that three iridium complexes had
the potential to inhibit biofilm formation and moderate
the ability to destroy pre-formed biofilm of S. aureus
ATCC 29,213. 250 lM concentration of synthesized
complexes showed the highest antibiofilm activity
(75% for Ir1, 90% for Ir2, and 71% for Ir3). The
significant inhibition obtained at 6.25 lM concentration
of Ir2 and Ir3 revealed the potential of our
samples. Also, Ir1 and Ir2 complexes had a good
capacity to destroy pre-formed biofilm. The results
clearly showed that iridium complexes have cytotoxic
activity towards colon cancer (Caco-2) and liver
cancer (HepG2) cell lines without affecting noncancerous
cells (HEK293) at applied doses. Moreover,
tested compounds induced apoptosis in these cancer
cells. All of these results showed that iridium
complexes had possessed the ability to inhibit or
destroy pre-formed biofilm and could be developed as
an effective agent against bacterial biofilms. Moreover,
these pure substances may have valuable anticancer
activity and it should be confirmed with further
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