عنوان مجله
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INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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چکیده
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Recently, specific interaction of anthrax protective antigen domain 4 (PAD4) and lethal factor domain 1 (LFD1)
have been considered for the design of novel diagnostic and therapeutic systems in medicine. In this study, theoretical
and experimental approaches were used to monitor the interactions of PAD4 and LFD1. CLusPro server
and Dimplot software were used to predict the interaction of these domains. Results, revealed interactive sites
between PAD4 and LFD1 on loop regions of both C and N terminal of PAD4. In experimental methods, PAD4
and LFD1were expressed in Escherichia coli and purified for usage inMagnetic Bead (MB) andMulti-Walled Carbon
Nanotubes (MWCNTs) based bio-sensing platforms. In the magnetic-based system, the magnetic sedimentation
of QD-PAD4 by MBs-LFD1 and the observation of the fluorescence spectrum related to QD-PAD4 in the
precipitated materials confirmed the interaction of PAD4 with LFD1 protein. In the MWCNTs-based method,
the QD-PAD4 fluorescence was quenched by absorption on MWCNTs. Upon the addition of LFD1, fluorescence
emission was recovered, indicating interaction of LFD1 with QD-PAD4, which results the separation of QDPAD4
from MWCNTs surfaces and fluorescence restoration. Finally, new approaches showed the interaction of
PAD4 and LFD1, which can be used as an attractive model in medicine.
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