کلیدواژهها
|
Antioxidant defense genes, In vitro, Nanoparticle, Salinity, Solanum Lycopersicon L
|
چکیده
|
Background: Salinity is one of the most challenging abiotic stresses restricting the growth of plants. In vitro screening
will increase the efficiency and speed of salinity tolerant genotypes identifications. The response of four tomato
cultivars under salinity was analyzed in vitro to evaluate the seedlings growth, biochemical, and gene expression
responses as well as the effect of nano zinc and iron on callus induction and plant regeneration.
Results: The results showed that an increase in salinity stress in the medium decreased the germination percentage,
fresh and dry weight of shoot, root length, chlorophyll a, b and carotenoids content, K and Ca content, and on the
other hand, Na content was increased. MDA content (‘Nora’, ‘PS-10’, ‘Peto’ and ‘Roma’: 1.71, 1.78, 1.66 and 2.16 folds,
respectively), electrolyte leakage (‘PS-10’: 33.33%; ‘Roma’: 56.33%), were increased with salinity of 100 mM compared
to control. Proline content was increased in 50 mM NaCl (10.8 fold). The most activity of antioxidant enzymes including
CAT, SOD, APX, GPX, and GR was observed in the ‘PS-10’ cultivar, and the lowest activity of these enzymes was
observed in ‘Roma’ under salinity stress. The AsA and GSH were decreased and DHA and GSSG were increased with the
increased intensity of salinity. The relative expression of SOD, APX, and GR genes varied in different cultivars at different
salinity concentrations. The most percentage of callus induction was observed with applying iron oxide nanoparticles,
and the most regeneration rate was recorded using zinc oxide nanoparticles.
Conclusion: The results showed that salt-tolerant cultivars such as ‘PS-10’ with better osmotic adjustment, are suitable
candidates for the future production and breeding programs.
|