عنوان مجله
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Pharmaceutical and Biomedical Research
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کلیدواژهها
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Isoniazid, Morphine
dependence, Naloxone,
Substance withdrawal
syndrome, Mice
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چکیده
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Background: GABAergic drugs have extensive interference with morphine’s pharmacological
effects, including dependence.
Objectives: The present study was conducted to evaluate the effects of isoniazid, a GABAergic
agent, on the acquisition and expression of morphine-induced dependence in male mice.
Methods: Sixty-four male mice were used. The mice became dependent on morphine by
administrating ten doses of morphine in four days. For the precipitation of the morphine
withdrawal signs (jumping, diarrhea, and weight loss), two hours after the last dose of
morphine, the mice received naloxone (4 mg/kg, IP). In the expression experiment, four
groups of mice received saline or isoniazid (25, 50, and 75 mg/kg, IP) one hour before
naloxone. In the acquisition experiment, the other four groups, one hour before the first six
doses of morphine, received saline or isoniazid (25, 50, and 75 mg/kg, IP).
Results: In the expression experiment, all doses of isoniazid decreased the number of
jumping in mice, but only the lowest dose influenced diarrhea (increased weight of diarrheal
stool) significantly. The higher doses of isoniazid (50 and 75 mg/kg, IP) caused a significant
reduction in the percentage of weight loss, but its lowest dose (25 mg/kg, IP) significantly
increased the sign. In the acquisition experiment, isoniazid (25, 50 mg/kg IP) decreased the
number of jumping and the percentage of weight loss, but not the weight of diarrheal stool.
Conclusion: Isoniazid may be a good candidate to prevent morphine withdrawal signs.
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