عنوان مجله
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International Journal of Health Science
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چکیده
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Objectives: The project is based on extensive studies on applied nanoparticles in
biology and medicine. This study was primarily designed to investigate the role of
magnetic nanoparticles by comparing the absorbance and stabilization of DNA-C, L
by the electrophoretic detection.
Methods: Fe3O4 magnetic nanoparticles (MNPs) with core/shell structure of silica coatings
were synthesized by a chemical coprecipitation method. This work is done at 15 min times
with multitimes, that is, 20 numbers and nanoparticles are provided regular with good
morphology which was synthesized in 20 nm in size, and its structure was analyzed by tools
such as EDX analysis. Next, 20 mg of the magnetic nanoparticles were coated with silica
in a heterogeneous solution at 25 μg/ml of the solution of each of the mixed DNAs (ring,
linear) in separate containers. Finally, 15 minutes later, DNA was adsorbed on the surface
of the nanoparticles. The amount of this adsorption was injected by spectrophotometry
(UV-Vis, ith 99% accuracy and optimized by the standard Tris.HCl buffer required to
separate DNA from its pure solution [unabsorbed DNA]) and electrophoresis.
Results: The results showed that absorption and diffusion of DNA-C or L at the surface
of nanoparticles were 95% and 85%, respectively (i.e., absorbance of DNA-C>DNA-L
is) with rate of removing of on MNPs was >99%. Hence, after review, we received
that a linkage of electrostatic bonding between nanoparticles and biomolecules was
obtained, and the results of the EDX analysis confirmed this study.
Conclusion: In this project, nanocomposites containing magnetic nanoparticles were
synthesized and their structure was identified by relevant analyzes. It was then used to
stabilize the biomolecules, which yielded competitive results between the two types
of DNA (linear and cyclic) at 85 and 95% adsorption, respectively.
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