عنوان مجله
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Ethiopian journal of health sciences
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چکیده
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BACKGROUND: In recent years, extensive studies have been
performed on magnetite nanoparticles (MNPs) and their
applications, which have shown the current project to be one of the
major applications by laboratory results.
METHODS: The nanoparticles synthesized in this project were
deposited by the co-precipitation method, which structure was
identified by analyzers such as SEM, FT-IR, and EDX. The aim of
this project is the adsorption and fixation of biomolecule (BSA
(bovine serum albumin) protein on the surface of magnetic
nanoparticles.
RESULTS: The adsorption results by electrophoresis and
spectrophotometric analyzers showed an absorption rate above
55% ie; 55% of the protein is fixed on the MNPs nanoparticles.
This absorption is due to the high level of functionality of magnetic
nanoparticles for adsorption of protein. The results of the EDX
analysis also show the possible electrostatic bonding between the
nanoparticles and the protein, this is derived from –OH with –NH2
groups of the nanobiocompound (MNPs /protein). After bonding,
the two are easily separated.
CONCLUSION: In this project, the Fe3 O4 nanoparticles was
synthesized and identified by SEM, FT-IR, and EDX analyzers and
finally reacted with the BSA protein (for the absorption of protein
on MNPs) under experimental conditions at a standard
temperature of 25° C. The results showed that about 55% of the
protein was fixed on magnetic nanoparticles.
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