عنوان مجله
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GENETIC RESOURCES AND CROP EVOLUTION
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چکیده
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The genetic diversity of 34 A. desertorum accessions was studied using 28 ISSR primers. A
total of 448 loci were amplifed in the A. desertorum
genome, and 402 (90%) of them were polymorphic.
The primer (AG)8YC produced the greatest number
of polymorphic fragments, while the primer (AC)8YA
produced the lowest number. The number of efective
alleles ranged between 1.26 and 1.57. The highest
value of Shanon index belonged to primer (GA)8T,
while the highest value of Nei index (0.33) was
detected for primers (GA)8T, (TC)8C, and (GACA)4.
Primer (GA)8GCC with PIC = 0.40 was the best informative marker in the evaluation of A. desertorum
genetic diversity. Genetic similarity among studied
accessions was between 0.45 (G03 and G17) and 0.80
(G01 and G11). Using Bayesian and WPGMA classifcation algorithms, the A. desertorum germplasm was
divided into two major subgroups (Red and Green),
which consisted of 13 and 17 accessions, respectively,
and also admixture accessions. Late-maturing accessions were distinguished from early-maturing accessions and mostly localized in the Green subgroup.
The Red subgroup had more private alleles as well
as heterozygosity compared with the Green subgroup
and therefore also had promising selection potential.
Totally, ISSR markers could distinguish early mature
genotypes, and this is signifcant for increased forage yield. Likewise, the recognized heterotic groups
could help breeders select highly polymorphic genotypes in polycross breeding.
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