| چکیده
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Aim: In the past decades, many efforts have been made with the aim of
searching for new tools to treat cancer. In this regard, the discovery,
investigation and application of techniques related to small interfering
RNAs (siRNA) has been one of the most significant advances in the field
of cancer detection and treatment. Small interfering RNA, sometimes
known as short interfering RNA or silencing RNA, is usually 21 bp long
and interferes with the expression of specific genes with complementary
nucleotide sequences and prevents translation by degrading mRNA after
transcription. Many studies have shown that siRNAs affect the regulation
of the expression of some genes that play a role in cancers. siRNAs are
effective on Snail transcription factors, which play an important role in the
invasion and metastasis of cancer cells, and miR-143, which plays an
important role in the pathogenesis of cancers. miRNAs together with
transcription factors can disrupt the biological pathways involved in
carcinogenesis. However, the exact effect of siRNA on the expression of
snail1 and miRNA-143 genes in breast cancer cells is not completely
clear. Based on this, the present study investigated the effects of siRNA
on snail1 and miRNA-143 on breast cancer cells.Material and Methods
were purchased from Pasteur Institute of Iran. The cells were cultured in
RPMI-1640 medium containing 10% FBS. Snail1 gene kit (Santacruz
biotechnology, California, USA) was used to treat cancer cells with
specific siRNA. The cells were divided into two groups: control (no
treatment) and treated cells (transfected with siRNA). In order to
determine the effective time, the cells were exposed to a dose of 60
picomoles of siRNA for 24, 48 and 72 hours. Beta actin gene was used as
internal control gene. Morphology of MDA-MB-468 metastatic cells were
examined using light microscopy before and after specific gene
transfection. Cell proliferation was checked by trypan blue staining.
Snail1 and miR-143 gene expression
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