چکیده
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In the present study, a simple, rapid, sensitive, and environmentally friendly method was proposed for the
determination of dobutamine hydrochloride (Dbn.HCl). The method is based on the inhibition of the
chemiluminescence (CL) arising from the reaction of luminol and graphene oxide (GO) in a strongly
alkaline medium by Dbn.HCl. GO was synthesized and fully characterized by various spectroscopic
methods, including TEM and SEM. The mechanisms of the generation and inhibition of CL were also
investigated and discussed. The experimental conditions were optimized and under optimum conditions,
the method showed a linear response for Dbn.HCl over the concentration range from 3.0 107 to
5.0 106 mol L1. The correlation coefficient (r2) of the calibration line was 0.9972, and the relative
standard deviation (RSD) of the method obtained by nine replicate determinations of 2.0 106 mol L1
Dbn.HCl was equal to 1.2%. The method was successfully used for the determination of Dbn.HCl in
various real samples.
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