چکیده
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A cursory survey of the literature shows that although peroxidase-like activity is a well-known and wellapplied
property of CuO nanoparticles (CuO NPs), the oxidase-like activity of CuO NPs is a property that
is somehow neglected to be incorporated in analytical applications. The present work reports that CuO
NPs, with intrinsic oxidase-like activity, catalyze the oxidation of luminol by dissolved oxygen (DO) to
generate enhanced chemiluminescence (CL) emission. In addition, it was found that ceftazidime (CFZM)
has an effective attenuating effect on the CuO NP-catalyzed luminol–O2 CL system in alkaline medium.
Based on these findings, a simple and sensitive CL method for the determination of CFZM was
developed. The linear dynamic range of the method was 1 107 to 4 106 mol L1 with a correlation
coefficient of the calibration line being r2 ¼ 0.9902. The limit of detection (LOD) was equal to 2.5 108
mol L1 and the relative standard deviation of the method with respect to 2.5 107 mol L1 CFZM was
equal to 4.5% (n ¼ 4). The applicability of the method was demonstrated for pharmaceutical
preparations, and tap and ground waters. The CuO NPs were synthesized by a quick-precipitation
method and their particle size distribution was analyzed. The synthesized CuO NPs were characterized
by using X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron
microscopy (TEM), and scanning electron microscopy (SEM). Dynamic light scattering (DLS) was also
used for analyzing the particle size-distribution of CuO NPs. In addition, the possible mechanism for the
CL reaction is discussed.
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