مشخصات پژوهش

عنوان	A Nano-Biosensor for the Detection of 185delAG Mutation in BRCA1 Gene, Leading to Breast Cancer
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Breast cancer; graphene oxide; 185delAG mutation; nano-biosensor; fluorescent
چکیده	Amethod was developed for the detection of 185delAG mutation in BRCA1 gene, which is responsible for 85% and 63% lifetime risks of hereditary breast and ovarian cancer in women, respectively. The protocol is based on the quenching and recovering fluorescence emission of fluorescein-based dye (FAM)-labeled DNA in the presence of graphene oxide (GO), followed by addition of cDNA or mDNA. In addition, ligase reaction between a DNA probe attached to GO and a DNA possessing FAM on 5 terminal in the presence of cDNA or mDNA was applied.
پژوهشگران	فرخ کریمی مندل بسر (نفر سوم)، ساجده کرمی نژاد (نفر دوم)، باقر افتخاری سیس (نفر اول)