مشخصات پژوهش

عنوان	Laccase production from sucrose by recombinant Yarrowia lipolytica
نوع پژوهش	مقاله ارائه شده کنفرانسی
کلیدواژه‌ها	Laccase, Yarrowia lipolytica, Trametes versicolor, Sucrose, Bioreactor.
چکیده	Background: Laccases are multi-copper enzymes that oxidized various aromatic and non-aromatic compounds and have diverse industrial applications from xenobiotic compounds degradation to biofuel production. The aim of this study was to investigate the effect of sucrose as a substrate on the laccase production by a recombinant strain of Yarrowia lipolytica in bioreactor. Methods: A batch culture of Y. lipolytica YL4 in 5 L bioreactor was carried out with 1.5 L of modified PPB medium containing 10% sucrose as a sole carbon source. The yeast cell growth was monitored by direct counting method on a haemocytometer. Laccase activity was determined spectrophotometrically by monitoring ABTS oxidation at 420 nm. Results: The laccase activity reached to 6.7 U/mL in bioreactor with modified PPB medium after 48 h which is 33.5 fold higher than PPB medium containing 2% glucose in the previous studies. Conclusion: Sucrose as a cost-effective and sole carbon source could be used to large scale production of laccase and other recombinant protein by Y. lipolytica.
پژوهشگران	کلاد جولیت (نفر چهارم)، کاترین مزدک (نفر سوم)، مرضیه مرادی (نفر اول)، فرشاد درویشی هرزویلی (نفر دوم)