مشخصات پژوهش

عنوان	Covalently bonded pancreatic lipase onto the dithiocarbamate/chitosan-based magnetite: Stepwise fabrication of Fe3O4@CS/NHCS-Lip as a novel and promising nanobiocatalyst
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Covalently enzyme immobilization Pancreatic lipase Nanobiocatalyst
چکیده	In the present paper, porcine pancreas lipase (PPL) was immobilized to a new version of magnetite via a novel stepwise dithiocarbamate/chitosan-based method in alternation to glutaraldehyde. Magnetic chitosan nanocomposite was post-modified to produce dithiocarbamate moieties on the surface through amine functions. Then, immobilization of lipase was successfully achieved on the surface of magnetically separable Fe3O4@CS/NHCS2H via a post-modification. Each step of immobilization was carefully monitored by characterization and all were successfully proved. Comparison of immobilized enzyme with free enzyme showed that the method of immobilization is efficient.
پژوهشگران	اسماعیل دوستخواه (نفر سوم)، محمد حیدری­زاده (نفر دوم)، صادق رستم نیا (نفر چهارم)، پریسا فتحی رضایی (نفر پنجم)، علی باغبان (نفر اول)