|
چکیده
|
Phylum nematoda includes the group of multicellular and vermiform animals, which found in all different habitats from poles to hot springs. Plant parasitic nematodes are jast 10% of the members of the phylum. The most destructive group of plant parasitis nematodes living in the plant so called endo parasites. the cabbage cyst nematode, Heterodera cruciferae Franklin,1945 is one of the plant endo parasitic nematode, that have been reported from different parts of the world and Iran, as well. Plant has specific active ingredients which can have role in prevention of diseases in humans can be considered as medicinal plant. The use of herbs to treat disease is almost universal among non-industrial societies and afton more affordable than buying chemical drugs. By investigation the parasitic nematodes in plant growing area, optimum usage of soil will be available. In this study, since main hosts of cabbage cyst nematode (kohlrabia, Brassica oleracea L. var. gongylodes, and white cabbage, Brassica oleracea L.var. captita alba) are naturally infected by cyst nematode in Tabriz vegetable growing areas and there is just generalization about the cabbage cyst nematode host rang in text book, in this study six medicand plant species namely Mlissa officinal, Lallemantia iberica, Dracocephalum moldavica, Descurainia sophia, Brassica alba and Brassic ajuncea infected by cabbage cyst nematode in greenhouse candition. After preparing the nematodes from vegetable growing area Tabriz, extraction of the nematodes (cyst) was performed using Fenwick 1940 and later or the cyst using needle and streomicroscope. Extracted cysts were sterilized by incubating in 0.6% sodium hypochlorde (Naocl) solution for 30 seconds. The soil was sterilized by autoclave and transferred to the greenhouse environment. Four to five cyst put in the vicinity of each seed in pots. In different stages from germination to flowering plants sampling carried on randomly and the roots were stained using a lactoglycerin-
|