|
چکیده
|
Introduction Respecting to over 170 million infected people worldwide, on today does not introduced any protective vaccine against Hepatitis C virus (HCV) infection and just 45% of patents can be treated by antiviral treatments [1]. Hepatitis C virus is a positive single strand RNA virus with 9.6 kb genomic length and is a member of Flaviviridea viral family. Genome of this virus codes a polypeptide with about 3000 amino acids which ultimately introduces P, E2, E1, C as structural proteins and Ns5b, Ns5a, Ns4b, Ns4a, Ns3, Ns2 as Non-structural proteins [2]. Within these proteins structural core protein (C) is a Multifunctional protein that play major role in viral capsid composition and also has important role pathogenesis via interfering in dendritic cells and interfering in lymphotoxin receptors and C1q which are important in T cell proliferation. likewise, Core protein is significant in apoptosis by interfering in Fas/TNF transgenic system. HCV infection diagnostic methods generally are based on detection of specific antibodies in serum. Main secreted antibodies against HCV are against Core protein thus Core protein is major candidate for diagnostic purposes [3,4]. Core protein has three domains that first domain (amino acid 2 to 122) is the hydrophilic part of the protein and correlated to viral RNA attachments for structural assembly [5]. Many viral biologic or pathophysiologic functions of HCV are acted by this domain [5]. Second domain (amino acid 123 to 174) is hydrophobic and has ability for contacting to lipid drops. Third domain (amino acid 174 to 192) separates from protein during the morphogenesis by cellular peptidases [5]. Mucosal immunity stimuli via the mouth and nasal because of their eath operation and ability to increase in immune protection in against mucosal pathogens is more Intended [6]. According to reports cholera toxin (Ctx) if be utilized with a recombinant vaccine together, as a strong mucosal adjuvant increases immune responses albeit i
|