مشخصات پژوهش

عنوان	Noncovalent immobilization of Yarrowia lipolytica lipase on Dendritic-like amino acid-functionalized silica nanoparticles
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Yarrowia lipolytica; lipase; biocatalysis; immobilization; dendrimer
چکیده	Immobilization of enzymes is a promising approach for the cost-e ective application of enzymes. Among others, noncovalent but unleachable approaches for immobilization are one of the most favorable and crucial approaches. Herein, silica nanoparticles are modified by (3-aminopropyl)triethoxysilane (APTES) to generate amino-functionalized silica nanoparticles. Then, the amine functionalities are converted to bifunctional amino acid via post-modification that has zwitterionic properties. This nanostructure with the new functional theme is employed to immobilize Yarrowia lipolytica lipase at room temperature with no further post-modification or cross-linking. This immobilization method is further compared with the metal chelate-based immobilization approach on the same support. The biocatalytic activity of the immobilized lipase is examined under various conditions. The encapsulation of lipase through amino acid-functionalized silica nanoparticles exhibited enhanced stability for the immobilized lipase at higher temperatures and unneutral pHs.
پژوهشگران	فرشاد درویشی هرزویلی (نفر چهارم)، غلامحسین ابراهیمی پور (نفر سوم)، اسماعیل دوستخواه (نفر دوم)، زهرا فتحی (نفر اول)