مشخصات پژوهش

صفحه نخست /Expression Profiling of ...
عنوان Expression Profiling of Candidate Genes in Sugar Beet Leaves Treated with Leonardite-Based Biostimulant
نوع پژوهش مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها biostimulant; BLACKJAK; gene expression profiling; humic acid; sugar beet
چکیده Leonardite-based biostimulants are a large class of compounds, including humic acid substances. Foliar application of biostimulants at field level improves plant growth, yield and quality through metabolic changes and stimulation of plant proton pumps. The present study aimed at identifying optimum dosage of BLACKJAK, a humic acid-based substance, which is able to modify genes involved in sugar beet growth. Thirty-three genes belonging to various biochemical pathwaycategoriesweretestedinleavesoftreatedsugarbeet(BetavulgarisL.)samplestoassessgene expression profiling in response to BLACKJAK. Seedlings of a diploid and multigerm variety were grown in plastic pots and sprayed with two dilutions of BLACKJAK (dilution 1:500–1.0 mg C L−1 and dilution 1:1000–0.5 mg C L−1). Leaf samples were collected after 24, 48, and 72 h treatment with BLACKJAK for each dilution. RNA was extracted and the quantification of gene expression was performed while using an OpenArray platform. Results of analysis of variance demonstrated that, 15 genes out of a total of 33 genes tested with OpenArray qPCR were significantly affected by treatment and exposure time. Analysis for annotation of gene products and pathways revealed that genes belongingto themitochondrial respiratorypathways, nitrogen and hormone metabolisms, and nutrient uptake were up-regulated in the BLACKJAK treated samples. Among the up-regulated genes, Bv_PHT2;1 and Bv_GLN1 expression exerted a 2-fold change in 1:1000 and 1:500 BLACKJAK concentrations. Overall, the gene expression data in the BLACKJAK treated leaves demonstrated the induction of plant growth–related genes that were contributed almost to amino acid and nitrogen metabolism, plant defense system, and plant growth.
پژوهشگران پیر جورجو استواناتو (نفر ششم به بعد)، سرنلا ناردی (نفر ششم به بعد)، جوزپه کون کری (نفر ششم به بعد)، جوانی کامپانیا (نفر ششم به بعد)، آندرا اسکوارتینی (نفر ششم به بعد)، ایوانا پولیسی (نفر ششم به بعد)، آندرا بالیه ری (نفر ششم به بعد)، کیارا بروکانلو (نفر ششم به بعد)، فرانچسکو ماگرو (نفر پنجم)، ماریا کریستینا دلا لوچیا (نفر چهارم)، جوانی برتولدو (نفر سوم)، بهرام حیدری (نفر دوم)، حنیفه سید حاجی زاده (نفر اول)