مشخصات پژوهش

عنوان	Complete genome sequence of Aphid lethal paralysis virus from metagenomic analysis of Cestrum elegans small RNAs
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Cestrum elegans Small RNA NGS ALPV
چکیده	Passive “vector” plants can harbor insect viruses without supporting their replication and may have epidemiological consequences. We report complete sequence of Aphid lethal paralysis virus (ALPV, Cripavirus, Dicistroviridae) from small RNA sequencing of a new passive “vector” plant, Cestrum elegans in Turkey using de novo assembly and annotating of contigs. Its genome is 9837 nt in length which encodes for 2 ORFs (including an intergenic untranslated region (IGR) of 196 nt) flanked by a 525 nt 5′ UTR and a 596 nt 3′ UTR. Multiple alignment and phylogenetic analysis showed that the isolate shares highest identity with ALPV-An (JX480861) isolated from oleander aphid, Aphis nerii and was placed into the same clade with other isolates of passive “vector” plants comprising cucumber and maize isolates.
پژوهشگران	شی فانگ لی (نفر چهارم)، مونا گازل (نفر سوم)، کادریه چاگلایان (نفر دوم)، وحید رومی (نفر اول)