مشخصات پژوهش

عنوان	Antibacterial activity of Lactobacillus rhamnosus cell-free supernatant against Staphylococcus aureus and Escherichia coli
نوع پژوهش	مقاله ارائه شده کنفرانسی
کلیدواژه‌ها	Lactobacillus rhamnosus, Escherichia coli, Staphylococcus aureus, Antibacterial activity
چکیده	Background and Aim : Probiotics may be a good alternative to antibiotics in fighting pathogenic bacteria. The current study aimed to evaluate the antibacterial activity of Lactobacillus rhamnosus cell-free supernatant against Staphylococcus aureus and Escherichia coli. Methods : L.􀀃 rhamnosus􀀃 ATCC􀀃 7469􀀃 was􀀃 cultured􀀃 in􀀃 the􀀃 MRS􀀃 broth􀀃 medium􀀃 at􀀃 37˚C.􀀃 The􀀃 medium was transferred to the tube after 48 h and centrifuged at 8000 rpm for 20 minutes. The supernatant was passed through a filter􀀃 with􀀃 0.2􀀃 μm􀀃 pores􀀃 under􀀃 sterile􀀃 conditions.􀀃 The􀀃 antibacterial activity of the filtered supernatant was determined against S. aureus ATCC 25923 and E. coli ATCC 8739 by minimum inhibitory concentration (MIC) method. Results : The minimum inhibitory concentration of L. rhamnosus cell-free supernatant against S. aureus and E. coli was 25 and 12.5 microliter/ml, respectively. Conclusion : The cell-free supernatant of L. rhamnosus had a good antibacterial effect on S. aureus and E. coli.
پژوهشگران	فرشاد درویشی هرزویلی (نفر چهارم)، بابک خلیفه زاده کلیبر (نفر سوم)، امیر عباس برزگری سرخه (نفر دوم)، سید علی علوی (نفر اول)