مشخصات پژوهش

عنوان	Identification of Pomegranate as a New Host of Passiflora Edulis Symptomless Virus (PeSV) and Analysis of PeSV Diversity
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Punica granatum; potyviridae; HTS; electron microscopy; RT-PCR
چکیده	Pomegranate is an important crop in the Mediterranean Basin that can be affected by a range of pathogens. With the aim to better understand the impact of viral diseases on pomegranate, two leaf samples from Turkey showing virus-like symptoms such as chlorotic spots and oak-leaf patterns were subjected to high throughput sequencing (HTS). Data analysis indicated the presence of passiflora edulis symptomless virus (PeSV: genus Roymovirus, Potyviridae family) in these two pomegranate samples, consistent with the observation by electron microscopy of flexuous filamentous viral particles 760 to 780 nm long. Further analysis of HTS reads revealed the presence of five PeSV variants in one of the samples and another single variant in the other. PeSV occurrence was also identified from publicly available SRA pomegranate RNA-Seq transcriptomic data from India and China. The genome of these PeSV-pomegranate variants share 78.0–86.8% nucleotide identity with that of the reference isolate from passionfruit (MH379332). The presence of PeSV in pomegranate was confirmed by specific RT-PCR assays targeting either the coat protein (CP) or Nla-Pro genes in 37 cultivated and one ornamental pomegranate out of 133 samples collected from the Eastern Mediterranean region of Turkey. To our knowledge, this is the first application of HTS to assess virus occurrence in pomegranate and the first recognition of pomegranate as a new host for PeSV.
پژوهشگران	تیری کندرسی (نفر ششم به بعد)، آنتونیو اولموش (نفر ششم به بعد)، آنا بلن روئیز گارسیا (نفر ششم به بعد)، ژان سباستین رینارد (نفر ششم به بعد)، بهار تونج (نفر پنجم)، حمیده دنیز کوجاباغ (نفر چهارم)، وحید رومی (نفر سوم)، مونا گازل (نفر دوم)، کادریه چاگلایان (نفر اول)