مشخصات پژوهش

عنوان	pH-responsive co-delivery of doxorubicin and saffron via cross-linked chitosan/laponite RD nanoparticles for enhanced-chemotherapy
نوع پژوهش	مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها	Co-delivery PH-responsive nanoparticles Doxorubicin Enhanced-chemotherap
چکیده	Combining natural antioxidants with synthetic anticancer agents is a helpful approach in chemotherapy to reduce side effects and enhance the effectiveness of drugs. Saffron extract (SAF), a natural antioxidant with anti-cancer properties, bears a protective effect against doxorubicin-induced toxicity. Co-delivery of doxorubicin (DOX), a known anticancer agent, and SAF using nanotechnology, can be helpful to achieve a highly effective chemotherapy result. This study aimed to design cross-linked chitosan/laponite RD (CS/LP®) nanoparticles for co-encapsulation of DOX and SAF. The results indicated a high encapsulation efficiency of DOX (≥83%) and SAF (≥60%) for synthesized nanocarriers. These carriers exhibited a highly controllable pH-responsive release behavior. The release of DOX and SAF remarkably increased by decreasing pH from neutral (pH 7.4) to acidic (pH 5.5). The effects of cross-linked CS content were evaluated on the release properties. An increase in cross-linked CS content imposed a sustained release behavior by decreasing the release rate. The kinetic studies were used to confirm the release mechanisms. In vitro tests performed high cytotoxicity for doxorubicin/saffron-loaded nanoparticles against MDA-MB-231 cells. The MTT assay demonstrated no cytotoxicity for CS/LP® nanoparticles confirming its biocompatibility. The DOX/SAF-loaded nanocarriers exhibited antibacterial activity against Gram-negative and Gram-positive bacteria.
پژوهشگران	حکیمه طاهری نژاد (نفر دوم)، سارا قاسم صلب (نفر ششم به بعد)، پریسا لبیب (نفر پنجم)، شهرزاد جوانشیر (نفر چهارم)، غلامرضا مهدوی نیا (نفر سوم)، باقر کاظمی (نفر اول)