مشخصات پژوهش

صفحه نخست /Combination of SIX4‑siRNA and ...
عنوان Combination of SIX4‑siRNA and temozolomide inhibits the growth and migration of A‑172 glioblastoma cancer cells
نوع پژوهش مقاله چاپ‌شده در مجلات علمی
کلیدواژه‌ها Glioblastoma · Temozolomide · SIX4-siRNA · Combination therapy · A-172 cell line
چکیده Glioblastoma is one of the most common and invasive types of primary brain malignancies in adults, accounting for 45.5% of malignancies. Its annual prevalence is low compared to other cancers. The survival rate of this disease is about 14 months after diagnosis. Temozolomide (TMZ) is a common chemotherapy drug used to treatment of glioblastoma, but drug resistance against this drug is an important barrier to successful treatment of this cancer. Today, siRNAs play a signifcant role in cancer treatment. SIX4 is a transcriptional regulatory molecule that can act as a transcriptional suppressor and an activator in target genes involved in diferentiation, migration, and cell survival processes. The aim of this study was to evaluate the efect of SIX4-siRNA on A-172 glioblastoma cells, its role as a tumor suppressor, and its combination with TMZ. We studied the cytotoxic efect of the SIX4-siRNA and TMZ on A-172 cells using the MTT assay investigated their efect on apoptosis and cell cycle of A-172 cells used wound healing assays to assess their efect on cell migration. Finally, we used qRT-PCR to study the mRNA expression levels of genes involved in apoptosis and migration of tumoral cells after treatments. Based on our results, silencing SIX4-siRNA expression reduced the cell viability of A-172 cells and sensitize these cells to TMZ. Furthermore, we observed an increase in apoptosis and cell cycle arrest, and a decrease in migration. Bax and caspase-9 overexpression and BCL2 and MMP9 downregulation were detected in the combination of SIX4-siRNA and TMZ. According to our results, the combination of SIX4-siRNA and TMZ can be a very useful strategy for successful glioblastoma treatment
پژوهشگران بهزاد برادران (نفر ششم به بعد)، نسا برپور (نفر ششم به بعد)، محمد امین دوست وندی (نفر پنجم)، امیر باغبانزاده (نفر چهارم)، دریا جواد رشبدی (نفر سوم)، رضا محمد زاده (نفر دوم)، زهرا جداری محمدپور (نفر اول)